2GRM

Crystal structure of PrgX/iCF10 complex

2GRM の概要

• エントリーDOI: 10.2210/pdb2grm/pdb
• 関連するPDBエントリー: 2GRL
• 分子名称: PrgX, peptide (3 entities in total)
• 機能のキーワード: receptor, inhibitor, transcription
• 由来する生物種: Enterococcus faecalis
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 112812.55

構造登録者

Shi, K.,Kozlowicz, B.K.,Gu, Z.Y.,Ohlendorf, D.H.,Earhart, C.A.,Dunny, G.M. (登録日: 2006-04-24, 公開日: 2007-04-03, 最終更新日: 2024-02-14)

主引用文献

Kozlowicz, B.K.,Shi, K.,Gu, Z.Y.,Ohlendorf, D.H.,Earhart, C.A.,Dunny, G.M.
Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptides.
Mol.Microbiol., 62:958-969, 2006

PubMed Abstract: In many bacteria expression of lateral gene transfer and of virulence factors is controlled by cell-cell signalling systems. Molecular interactions of microbial signal molecules with their cognate receptors are not well understood. For the Enterococcus faecalis conjugative plasmid pCF10, the PrgX protein serves as a molecular switch controlling expression of conjugation and virulence genes encoded by the plasmid. The induction state of a pCF10-carrying donor cell is determined by the ratio of two signalling peptides, cCF10 pheromone and iCF10 inhibitor. Recent analysis of PrgX/cCF10 interactions suggests a mechanism for conversion to the induced state. However, the means by which iCF10 peptide antagonizes cCF10 activity is unclear, and it has been suggested that inhibitor peptides block import of pheromone peptides. We now show that both of these peptides interact with the same binding pocket of PrgX, but they differentially alter the conformation of the protein and its oligomerization state, resulting in opposing biological activities.

PubMed: 17038121
DOI: 10.1111/j.1365-2958.2006.05434.x

実験手法

X-RAY DIFFRACTION (3 Å)