2GR0

Crystal structure of Ferredoxin reductase, BphA4 (oxidized form, NAD+ complex)

2GR0 の概要

• エントリーDOI: 10.2210/pdb2gr0/pdb
• 関連するPDBエントリー: 2GQW 2GQY 2GQZ 2GR1 2GR2 2GR3
• 分子名称: ferredoxin reductase, FLAVIN-ADENINE DINUCLEOTIDE, ADENOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: flavoprotein, oxidoreductase
• 由来する生物種: Pseudomonas sp. KKS102
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44433.94

構造登録者

Senda, T.,Senda, M. (登録日: 2006-04-22, 公開日: 2007-05-22, 最終更新日: 2024-03-13)

主引用文献

Senda, M.,Kishigami, S.,Kimura, S.,Fukuda, M.,Ishida, T.,Senda, T.
Molecular Mechanism of the Redox-dependent Interaction between NADH-dependent Ferredoxin Reductase and Rieske-type [2Fe-2S] Ferredoxin
J.Mol.Biol., 373:382-400, 2007

PubMed Abstract: The electron transfer system of the biphenyl dioxygenase BphA, which is derived from Acidovorax sp. (formally Pseudomonas sp.) strain KKS102, is composed of an FAD-containing NADH-ferredoxin reductase (BphA4) and a Rieske-type [2Fe-2S] ferredoxin (BphA3). Biochemical studies have suggested that the whole electron transfer process from NADH to BphA3 comprises three consecutive elementary electron-transfer reactions, in which BphA3 and BphA4 interact transiently in a redox-dependent manner. Initially, BphA4 receives two electrons from NADH. The reduced BphA4 then delivers one electron each to the [2Fe-2S] cluster of the two BphA3 molecules through redox-dependent transient interactions. The reduced BphA3 transports the electron to BphA1A2, a terminal oxygenase, to support the activation of dioxygen for biphenyl dihydroxylation. In order to elucidate the molecular mechanisms of the sequential reaction and the redox-dependent interaction between BphA3 and BphA4, we determined the crystal structures of the productive BphA3-BphA4 complex, and of free BphA3 and BphA4 in all the redox states occurring in the catalytic cycle. The crystal structures of these reaction intermediates demonstrated that each elementary electron transfer induces a series of redox-dependent conformational changes in BphA3 and BphA4, which regulate the interaction between them. In addition, the conformational changes induced by the preceding electron transfer seem to induce the next electron transfer. The interplay of electron transfer and induced conformational changes seems to be critical to the sequential electron-transfer reaction from NADH to BphA3.

PubMed: 17850818
DOI: 10.1016/j.jmb.2007.08.002

実験手法

X-RAY DIFFRACTION (1.7 Å)