2GO5

Structure of signal recognition particle receptor (SR) in complex with signal recognition particle (SRP) and ribosome nascent chain complex

2GO5 の概要

• エントリーDOI: 10.2210/pdb2go5/pdb
• EMDBエントリー: 1217
• 分子名称: SRP RNA, ribosomal RNA, Signal recognition particle 19 kDa protein (SRP19), ... (9 entities in total)
• 機能のキーワード: sr, srp, ribosome, translation-rna complex, translation/rna
• 由来する生物種: Canis sp.; 詳細
• 細胞内の位置: Cytoplasm: P61010; Endoplasmic reticulum membrane; Peripheral membrane protein: P08240; Endoplasmic reticulum membrane; Single-pass membrane protein (By similarity): P47758
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 185745.28

構造登録者

Halic, M.,Gartmann, M.,Schlenker, O.,Mielke, T.,Pool, M.R.,Sinning, I.,Beckmann, R. (登録日: 2006-04-12, 公開日: 2006-06-13, 最終更新日: 2024-10-09)

主引用文献

Halic, M.,Gartmann, M.,Schlenker, O.,Mielke, T.,Pool, M.R.,Sinning, I.,Beckmann, R.
Signal Recognition Particle Receptor Exposes the Ribosomal Translocon Binding Site
Science, 312:745-747, 2006

PubMed Abstract: Signal sequences of secretory and membrane proteins are recognized by the signal recognition particle (SRP) as they emerge from the ribosome. This results in their targeting to the membrane by docking with the SRP receptor, which facilitates transfer of the ribosome to the translocon. Here, we present the 8 angstrom cryo-electron microscopy structure of a "docking complex" consisting of a SRP-bound 80S ribosome and the SRP receptor. Interaction of the SRP receptor with both SRP and the ribosome rearranged the S domain of SRP such that a ribosomal binding site for the translocon, the L23e/L35 site, became exposed, whereas Alu domain-mediated elongation arrest persisted.

PubMed: 16675701
DOI: 10.1126/science.1124864

実験手法

ELECTRON MICROSCOPY (7.4 Å)