2GMP

Metal-free (apo) P. angolensis seed lectin in complex with GlcNAC-beta(1-2)Man

2GMP の概要

• エントリーDOI: 10.2210/pdb2gmp/pdb
• 関連するPDBエントリー: 1UKG 2GME 2GMM 2GN3 2GNB
• 分子名称: lectin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: legume lectin, metal-free lectin, sugar complex, glcnac-beta(1-2)man, beta sandwich, sugar binding protein
• 由来する生物種: Pterocarpus angolensis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 55979.35

構造登録者

Garcia-Pino, A.,Buts, L.,Wyns, L.,Loris, R. (登録日: 2006-04-07, 公開日: 2006-07-25, 最終更新日: 2024-10-30)

主引用文献

Garcia-Pino, A.,Buts, L.,Wyns, L.,Loris, R.
Interplay Between Metal Binding and cis/trans Isomerization in Legume Lectins: Structural and Thermodynamic Study of P. angolensis Lectin.
J.Mol.Biol., 361:153-167, 2006

PubMed Abstract: The interplay between metal binding, carbohydrate binding activity, stability and structure of the lectin from Pterocarpus angolensis was investigated. Removal of the metals leads to a more flexible form of the protein with significantly less conformational stability. Crystal structures of this metal-free form show significant structural rearrangements, although some structural features that allow the binding of sugars are retained. We propose that substitution of an asparagine residue at the start of the C-terminal beta-strand of the legume lectin monomer hinders the trans-isomerization of the cis-peptide bond upon demetallization and constitutes an intramolecular switch governing the isomer state of the non-proline bond and ultimately the lectin phenotype.

PubMed: 16824540
DOI: 10.1016/j.jmb.2006.06.006

実験手法

X-RAY DIFFRACTION (2.5 Å)