2GML

Crystal Structure of Catalytic Domain of E.coli RluF

2GML の概要

• エントリーDOI: 10.2210/pdb2gml/pdb
• 分子名称: Ribosomal large subunit pseudouridine synthase F (2 entities in total)
• 機能のキーワード: pseudouridine synthase, rluf, ribosome, rna modifying enzyme, isomerase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 53635.01

構造登録者

Sunita, S.,Zhenxing, H.,Swaathi, J.,Cygler, M.,Matte, A.,Sivaraman, J. (登録日: 2006-04-06, 公開日: 2006-07-18, 最終更新日: 2024-11-13)

主引用文献

Sunita, S.,Zhenxing, H.,Swaathi, J.,Cygler, M.,Matte, A.,Sivaraman, J.
Domain Organization and Crystal Structure of the Catalytic Domain of E.coli RluF, a Pseudouridine Synthase that Acts on 23S rRNA
J.Mol.Biol., 359:998-1009, 2006

PubMed Abstract: Pseudouridine synthases catalyze the isomerization of uridine to pseudouridine (Psi) in rRNA and tRNA. The pseudouridine synthase RluF from Escherichia coli (E.C. 4.2.1.70) modifies U2604 in 23S rRNA, and belongs to a large family of pseudouridine synthases present in all kingdoms of life. Here we report the domain architecture and crystal structure of the catalytic domain of E.coli RluF at 2.6A resolution. Limited proteolysis, mass spectrometry and N-terminal sequencing indicate that RluF has a distinct domain architecture, with the catalytic domain flanked at the N and C termini by additional domains connected to it by flexible linkers. The structure of the catalytic domain of RluF is similar to those of RsuA and TruB. RluF is a member of the RsuA sequence family of Psi-synthases, along with RluB and RluE. Structural comparison of RluF with its closest structural homologues, RsuA and TruB, suggests possible functional roles for the N-terminal and C-terminal domains of RluF.

PubMed: 16712869
DOI: 10.1016/j.jmb.2006.04.019

実験手法

X-RAY DIFFRACTION (2.6 Å)