2GLI

FIVE-FINGER GLI/DNA COMPLEX

Summary for 2GLI

• Entry DOI: 10.2210/pdb2gli/pdb
• Descriptor: DNA (5'-D(*TP*TP*TP*CP*GP*TP*CP*TP*TP*GP*GP*GP*TP*GP*GP*TP*CP*CP*AP*CP*G)-3'), DNA (5'-D(*AP*CP*GP*TP*GP*GP*AP*CP*CP*AP*CP*CP*CP*AP*AP*GP*AP*CP*GP*AP*A)-3'), PROTEIN (FIVE-FINGER GLI), ... (5 entities in total)
• Functional Keywords: protein-dna complex, transcription-dna complex, transcription/dna
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm: P08151
• Total number of polymer chains: 3
• Total formula weight: 31372.59

Authors

Pavletich, N.P.,Pabo, C.O. (deposition date: 1993-11-09, release date: 1993-11-09, Last modification date: 2024-02-14)

Primary citation

Pavletich, N.P.,Pabo, C.O.
Crystal structure of a five-finger GLI-DNA complex: new perspectives on zinc fingers.
Science, 261:1701-1707, 1993

PubMed Abstract: Zinc finger proteins, of the type first discovered in transcription factor IIIA (TFIIIA), are one of the largest and most important families of DNA-binding proteins. The crystal structure of a complex containing the five Zn fingers from the human GLI oncogene and a high-affinity DNA binding site has been determined at 2.6 A resolution. Finger one does not contact the DNA. Fingers two through five bind in the major groove and wrap around the DNA, but lack the simple, strictly periodic arrangement observed in the Zif268 complex. Fingers four and five of GLI make extensive base contacts in a conserved nine base-pair region, and this section of the DNA has a conformation intermediate between B-DNA and A-DNA. Analyzing the GLI complex and comparing it with Zif268 offers new perspectives on Zn finger-DNA recognition.

PubMed: 8378770

Experimental method

X-RAY DIFFRACTION (2.6 Å)