2GLI

FIVE-FINGER GLI/DNA COMPLEX

2GLI の概要

• エントリーDOI: 10.2210/pdb2gli/pdb
• 分子名称: DNA (5'-D(*TP*TP*TP*CP*GP*TP*CP*TP*TP*GP*GP*GP*TP*GP*GP*TP*CP*CP*AP*CP*G)-3'), DNA (5'-D(*AP*CP*GP*TP*GP*GP*AP*CP*CP*AP*CP*CP*CP*AP*AP*GP*AP*CP*GP*AP*A)-3'), PROTEIN (FIVE-FINGER GLI), ... (5 entities in total)
• 機能のキーワード: protein-dna complex, transcription-dna complex, transcription/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P08151
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 31372.59

構造登録者

Pavletich, N.P.,Pabo, C.O. (登録日: 1993-11-09, 公開日: 1993-11-09, 最終更新日: 2024-02-14)

主引用文献

Pavletich, N.P.,Pabo, C.O.
Crystal structure of a five-finger GLI-DNA complex: new perspectives on zinc fingers.
Science, 261:1701-1707, 1993

PubMed Abstract: Zinc finger proteins, of the type first discovered in transcription factor IIIA (TFIIIA), are one of the largest and most important families of DNA-binding proteins. The crystal structure of a complex containing the five Zn fingers from the human GLI oncogene and a high-affinity DNA binding site has been determined at 2.6 A resolution. Finger one does not contact the DNA. Fingers two through five bind in the major groove and wrap around the DNA, but lack the simple, strictly periodic arrangement observed in the Zif268 complex. Fingers four and five of GLI make extensive base contacts in a conserved nine base-pair region, and this section of the DNA has a conformation intermediate between B-DNA and A-DNA. Analyzing the GLI complex and comparing it with Zif268 offers new perspectives on Zn finger-DNA recognition.

PubMed: 8378770

実験手法

X-RAY DIFFRACTION (2.6 Å)