2GL2

Crystal structure of the tetra mutant (T66G,R67G,F68G,Y69G) of bacterial adhesin FadA

2GL2 の概要

• エントリーDOI: 10.2210/pdb2gl2/pdb
• 関連するPDBエントリー: 2AVR 2gkq 2gld
• 分子名称: adhesion A (2 entities in total)
• 機能のキーワード: antiparallel helix-loop-helix, fada gly4 mutant, cell adhesion
• 由来する生物種: Fusobacterium nucleatum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26658.80

構造登録者

Nithianantham, S.,Xu, M.,Wu, N.,Shoham, M.,Han, Y.W. (登録日: 2006-04-04, 公開日: 2007-04-10, 最終更新日: 2023-11-15)

主引用文献

Nithianantham, S.,Xu, M.,Wu, N.,Han, Y.W.,Shoham, M.
Crystallization and preliminary X-ray data of the FadA adhesin from Fusobacterium nucleatum.
Acta Crystallogr.,Sect.F, 62:1215-1217, 2006

PubMed Abstract: Fusobacterium nucleatum is a gram-negative anaerobe prevalent in the oral cavity that is associated with periodontal disease, preterm birth and infections in other parts of the human body. The bacteria attach to and invade epithelial and endothelial cells in the gum tissue and elsewhere via a 13.7 kDa adhesin protein FadA (Fusobacterium adhesin A). FadA exists in two forms: the intact form (pre-FadA), consisting of 129 amino acids, and the mature form (mFadA), which lacks an 18-residue signal sequence. Both forms have been expressed in Escherichia coli and purified. mFadA has been crystallized. The crystals belong to the hexagonal space group P6(1) or P6(5), with unit-cell parameters a = b = 59.3, c = 125.7 A and one molecule per asymmetric unit. The crystals exhibit an unusually high solvent content of 74%. Synchrotron X-ray data have been collected to 1.9 A. The crystals are suitable for X-ray structure determination. The crystal structure of FadA may provide a basis for the development of therapeutic agents to combat periodontal disease and other infections associated with F. nucleatum.

PubMed: 17142900
DOI: 10.1107/S1744309106045593

実験手法

X-RAY DIFFRACTION (2.5 Å)