2GIP

Solution structure of a portion of the 5'UTR of HspA mRNA from Bradyrhizobium janponicum having deleted G83

2GIP の概要

• エントリーDOI: 10.2210/pdb2gip/pdb
• 分子名称: 28-MER (1 entity in total)
• 機能のキーワード: a form rna helix, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8901.25

構造登録者

Chowdhury, S.,Maris, C.,Allain, F.H.,Narberhaus, F. (登録日: 2006-03-29, 公開日: 2006-06-20, 最終更新日: 2024-05-29)

主引用文献

Chowdhury, S.,Maris, C.,Allain, F.H.,Narberhaus, F.
Molecular basis for temperature sensing by an RNA thermometer.
Embo J., 25:2487-2497, 2006

PubMed Abstract: Regulatory RNA elements, like riboswitches, respond to intracellular signals by three-dimensional (3D) conformational changes. RNA thermometers employ a similar strategy to sense temperature changes in the cell and regulate the translational machinery. We present here the first 3D NMR structure of the functional domain of a highly conserved bacterial RNA thermometer containing the ribosome binding site that remains occluded at normal temperatures (30 degrees C). We identified a region adjacent to the Shine-Dalgarno sequence that has a network of weak hydrogen bonds within the RNA helix. With the onset of heat shock at 42 degrees C, destabilisation of the RNA structure initiates at this region and favours the release of the ribosome binding site and of the start codon. Deletion of a highly conserved G residue leads to the formation of a stable regular RNA helix that loses thermosensing ability. Our results indicate that RNA thermometers are able to sense temperature changes without the aid of accessory factors.

PubMed: 16710302
DOI: 10.1038/sj.emboj.7601128

実験手法

SOLUTION NMR