2GIO
Solution Structure of a portion of the 5'UTR of HspA mRNA of Bradyrhizobium japonicum
Summary for 2GIO
| Entry DOI | 10.2210/pdb2gio/pdb |
| Descriptor | 29-MER (1 entity in total) |
| Functional Keywords | g-g non cannonical pair, rna |
| Total number of polymer chains | 1 |
| Total formula weight | 9246.46 |
| Authors | Chowdhury, S.,Maris, C.,Allain, F.H.,Narberhaus, F. (deposition date: 2006-03-29, release date: 2006-06-20, Last modification date: 2024-05-29) |
| Primary citation | Chowdhury, S.,Maris, C.,Allain, F.H.,Narberhaus, F. Molecular basis for temperature sensing by an RNA thermometer. Embo J., 25:2487-2497, 2006 Cited by PubMed Abstract: Regulatory RNA elements, like riboswitches, respond to intracellular signals by three-dimensional (3D) conformational changes. RNA thermometers employ a similar strategy to sense temperature changes in the cell and regulate the translational machinery. We present here the first 3D NMR structure of the functional domain of a highly conserved bacterial RNA thermometer containing the ribosome binding site that remains occluded at normal temperatures (30 degrees C). We identified a region adjacent to the Shine-Dalgarno sequence that has a network of weak hydrogen bonds within the RNA helix. With the onset of heat shock at 42 degrees C, destabilisation of the RNA structure initiates at this region and favours the release of the ribosome binding site and of the start codon. Deletion of a highly conserved G residue leads to the formation of a stable regular RNA helix that loses thermosensing ability. Our results indicate that RNA thermometers are able to sense temperature changes without the aid of accessory factors. PubMed: 16710302DOI: 10.1038/sj.emboj.7601128 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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