2GI7

Crystal structure of human platelet Glycoprotein VI (GPVI)

Summary for 2GI7

• Entry DOI: 10.2210/pdb2gi7/pdb
• Descriptor: GPVI protein, SULFATE ION, GLYCEROL, ... (5 entities in total)
• Functional Keywords: ig-like domains, blood clotting, cell adhesion
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 40811.44

Authors

Horii, K.,Kahn, M.L.,Herr, A.B. (deposition date: 2006-03-28, release date: 2007-04-03, Last modification date: 2024-11-06)

Primary citation

Horii, K.,Kahn, M.L.,Herr, A.B.
Structural basis for platelet collagen responses by the immune-type receptor glycoprotein VI.
Blood, 108:936-942, 2006

PubMed Abstract: Activation of circulating platelets by exposed vessel wall collagen is a primary step in the pathogenesis of heart attack and stroke, and drugs to block platelet activation have successfully reduced cardiovascular morbidity and mortality. In humans and mice, collagen activation of platelets is mediated by glycoprotein VI (GPVI), a receptor that is homologous to immune receptors but bears little sequence similarity to known matrix protein adhesion receptors. Here we present the crystal structure of the collagen-binding domain of human GPVI and characterize its interaction with a collagen-related peptide. Like related immune receptors, GPVI contains 2 immunoglobulin-like domains arranged in a perpendicular orientation. Significantly, GPVI forms a back-to-back dimer in the crystal, an arrangement that could explain data previously obtained from cell-surface GPVI inhibition studies. Docking algorithms identify 2 parallel grooves on the GPVI dimer surface as collagen-binding sites, and the orientation and spacing of these grooves precisely match the dimensions of an intact collagen fiber. These findings provide a structural basis for the ability of an immune-type receptor to generate signaling responses to collagen and for the development of GPVI inhibitors as new therapies for human cardiovascular disease.

PubMed: 16861347
DOI: 10.1182/blood-2006-01-010215

Experimental method

X-RAY DIFFRACTION (2.4 Å)