2GCC

SOLUTION STRUCTURE OF THE GCC-BOX BINDING DOMAIN, NMR, MINIMIZED MEAN STRUCTURE

2GCC の概要

• エントリーDOI: 10.2210/pdb2gcc/pdb
• 分子名称: ATERF1 (1 entity in total)
• 機能のキーワード: transcription factor, ethlene inducible
• 由来する生物種: Arabidopsis thaliana (thale cress)
• 細胞内の位置: Nucleus (Probable): O80337
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7891.97

構造登録者

Allen, M.D.,Yamasaki, K.,Ohme-Takagi, M.,Tateno, M.,Suzuki, M. (登録日: 1998-03-13, 公開日: 1999-03-23, 最終更新日: 2024-05-29)

主引用文献

Allen, M.D.,Yamasaki, K.,Ohme-Takagi, M.,Tateno, M.,Suzuki, M.
A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA.
EMBO J., 17:5484-5496, 1998

PubMed Abstract: The 3D solution structure of the GCC-box binding domain of a protein from Arabidopsis thaliana in complex with its target DNA fragment has been determined by heteronuclear multidimensional NMR in combination with simulated annealing and restrained molecular dynamic calculation. The domain consists of a three-stranded anti-parallel beta-sheet and an alpha-helix packed approximately parallel to the beta-sheet. Arginine and tryptophan residues in the beta-sheet are identified to contact eight of the nine consecutive base pairs in the major groove, and at the same time bind to the sugar phosphate backbones. The target DNA bends slightly at the central CG step, thereby allowing the DNA to follow the curvature of the beta-sheet.

PubMed: 9736626
DOI: 10.1093/emboj/17.18.5484

実験手法

SOLUTION NMR