2GAT

SOLUTION STRUCTURE OF THE C-TERMINAL DOMAIN OF CHICKEN GATA-1 BOUND TO DNA, NMR, REGULARIZED MEAN STRUCTURE

2GAT の概要

• エントリーDOI: 10.2210/pdb2gat/pdb
• 分子名称: DNA (5'-D(*GP*TP*TP*GP*CP*AP*GP*AP*TP*AP*AP*AP*CP*AP*TP*T)-3'), DNA (5'-D(*AP*AP*TP*GP*TP*TP*TP*AP*TP*CP*TP*GP*CP*AP*AP*C)-3'), ERYTHROID TRANSCRIPTION FACTOR GATA-1, ... (4 entities in total)
• 機能のキーワード: dna binding protein, transcription factor, zinc binding domain, complex (transcription regulation-dna), transcription-dna complex, transcription/dna
• 由来する生物種: Gallus gallus (chicken)
• 細胞内の位置: Nucleus: P17678
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 17416.67

構造登録者

Clore, G.M.,Tjandra, N.,Starich, M.,Omichinski, J.G.,Gronenborn, A.M. (登録日: 1997-11-07, 公開日: 1998-01-28, 最終更新日: 2024-05-29)

主引用文献

Tjandra, N.,Omichinski, J.G.,Gronenborn, A.M.,Clore, G.M.,Bax, A.
Use of dipolar 1H-15N and 1H-13C couplings in the structure determination of magnetically oriented macromolecules in solution.
Nat.Struct.Biol., 4:732-738, 1997

PubMed Abstract: Anisotropy of the molecular magnetic susceptibility gives rise to a small degree of alignment. The resulting residual dipolar couplings, which can now be measured with the advent of higher magnetic fields in NMR, contain information on the orientation of the internuclear vectors relative to the molecular magnetic susceptibility tensor, thereby providing information on long range order that is not accessible by any of the solution NMR parameters currently used in structure determination. Thus, the dipolar couplings constitute unique and powerful restraints in determining the structures of magnetically oriented macromolecules in solution. The method is demonstrated on a complex of the DNA-binding domain of the transcription factor GATA-1 with a 16 base pair oligodeoxyribonucleotide.

PubMed: 9303001
DOI: 10.1038/nsb0997-732

実験手法

SOLUTION NMR