2GAA

Crystal structure of YFH7 from Saccharomyces cerevisiae: a putative P-loop containing kinase with a circular permutation.

Summary for 2GAA

• Entry DOI: 10.2210/pdb2gaa/pdb
• Related: 1UJ2 2GA8
• Descriptor: Hypothetical 39.9 kDa protein, SULFATE ION (3 entities in total)
• Functional Keywords: yfr007w, yfh7, unknown function
• Biological source: Saccharomyces cerevisiae (baker's yeast)
• Total number of polymer chains: 1
• Total formula weight: 41092.70

Authors

Chaptal, V.,Morera, S. (deposition date: 2006-03-08, release date: 2007-03-27, Last modification date: 2024-10-30)

Primary citation

Gueguen-Chaignon, V.,Chaptal, V.,Lariviere, L.,Costa, N.,Lopes, P.,Morera, S.,Nessler, S.
Crystal structure and functional analysis identify the P-loop containing protein YFH7 of Saccharomyces cerevisiae as an ATP-dependent kinase.
Proteins, 71:804-812, 2007

PubMed Abstract: Genome sequencing projects have revealed that P-loop proteins are highly represented in all organisms and that many of them have no attributed function. They are characterized by a conserved nucleotide-binding domain and carry different activities implicated in many cellular processes. Saccharomyces cerevisiae YFH7 is one of these P-loop proteins of unknown function. In this work we tried to integrate bioinformatics, structure, and enzymology to discover the function of YFH7. Sequence analysis revealed that yeast YFH7 is a yeast-specific protein showing weak similarity with the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop containing kinases. A large insertion of about 100 residues distinguishes YFH7 from other members of the family. The 1.95 A resolution crystal structure of YFH7 solved using the SAD method confirmed that YFH7 has a fold similar to the PRK/URK/PANK family, with the characteristic core, lid, and NMP(bind) domains. An additional alpha/beta domain of novel topology corresponds to the large sequence insertion. Structural and ligand binding analysis combined with enzymatic assays suggest that YFH7 is an ATP-dependent small molecule kinase with new substrate specificity.

PubMed: 18004758
DOI: 10.1002/prot.21740

Experimental method

X-RAY DIFFRACTION (1.95 Å)