2GA7

Solution structure of the copper(I) form of the third metal-binding domain of ATP7A protein (menkes disease protein)

2GA7 の概要

• エントリーDOI: 10.2210/pdb2ga7/pdb
• 関連するPDBエントリー: 2G9O
• NMR情報: BMRB: 7068
• 分子名称: Copper-transporting ATPase 1, COPPER (I) ION (2 entities in total)
• 機能のキーワード: solution structure, menkes disease-associated protein, copper(i), structural genomics, structural proteomics in europe, spine, hydrolase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Isoform 3: Cytoplasm, cytosol (Probable). Isoform 5: Endoplasmic reticulum: Q04656
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9936.58

構造登録者

Banci, L.,Bertini, I.,Cantini, F.,DellaMalva, N.,Rosato, A.,Herrmann, T.,Wuthrich, K.,Structural Proteomics in Europe (SPINE) (登録日: 2006-03-08, 公開日: 2006-08-01, 最終更新日: 2024-05-29)

主引用文献

Banci, L.,Bertini, I.,Cantini, F.,DellaMalva, N.,Herrmann, T.,Rosato, A.,Wuthrich, K.
Solution structure and intermolecular interactions of the third metal-binding domain of ATP7A, the Menkes disease protein.
J.Biol.Chem., 281:29141-29147, 2006

PubMed Abstract: The third metal-binding domain of the human Menkes protein (MNK3), a copper(I)-transporting ATPase, has been expressed in Escherichia coli and characterized in solution. The solution structure of MNK3, its copper(I)-binding properties, and its interaction with the physiological partner, HAH1, have been studied. MNK3 is the domain most dissimilar in structure from the other domains of the Menkes protein. This is reflected in a significant rearrangement of the last strand of the four-stranded beta-sheet when compared with the other known homologous proteins or protein domains. MNK3 is also peculiar with respect to its interaction with the copper(I) ion, as it was found to be a comparatively weak binder. Copper(I) transfer from metal-loaded HAH1 was observed experimentally, but the metal distribution was shifted toward binding by HAH1. This is at variance with what is observed for the other Menkes domains.

PubMed: 16873374
DOI: 10.1074/jbc.M603176200

実験手法

SOLUTION NMR