2G7C

Clostridium difficile Toxin A Fragment Bound to aGal(1,3)bGal(1,4)bGlcNAc

Summary for 2G7C

• Entry DOI: 10.2210/pdb2g7c/pdb
• Related: 2F6E
• Descriptor: Toxin A, alpha-D-galactopyranose-(1-3)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, GLYCEROL, ... (4 entities in total)
• Functional Keywords: linear b trisaccharide, protein-carbohydrate complex, bacterial toxin, toxin
• Biological source: Clostridium difficile
• Total number of polymer chains: 2
• Total formula weight: 60024.00

Authors

Greco, A.,Ho, J.G.S.,Lin, S.J.,Palcic, M.M.,Rupnik, M.,Ng, K.K.S. (deposition date: 2006-02-28, release date: 2006-04-18, Last modification date: 2023-09-20)

Primary citation

Greco, A.,Ho, J.G.,Lin, S.J.,Palcic, M.M.,Rupnik, M.,Ng, K.K.
Carbohydrate recognition by Clostridium difficile toxin A.
Nat.Struct.Mol.Biol., 13:460-461, 2006

PubMed Abstract: Clostridium difficile TcdA is a large toxin that binds carbohydrates on intestinal epithelial cells. A 2-A resolution cocrystal structure reveals two molecules of alpha-Gal-(1,3)-beta-Gal-(1,4)-beta-GlcNAcO(CH(2))(8)CO(2)CH(3) binding in an extended conformation to TcdA. Residues forming key contacts with the trisaccharides are conserved in all seven putative binding sites in TcdA, suggesting a mode of multivalent binding that may be exploited for the rational design of novel therapeutics.

PubMed: 16622409
DOI: 10.1038/nsmb1084

Experimental method

X-RAY DIFFRACTION (2 Å)