2G6X

Crystal structure of a novel green fluorescent protein from marine copepod Pontellina plumata

2G6X の概要

• エントリーDOI: 10.2210/pdb2g6x/pdb
• 関連するPDBエントリー: 2G6Y
• 分子名称: green fluorescent protein 2 (2 entities in total)
• 機能のキーワード: green fluorescent protein, natural chromophore, rapid maturation, beta can, luminescent protein
• 由来する生物種: Pontellina plumata
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 97576.69

構造登録者

Evdokimov, A.G.,Pokross, M.E.,Chudakov, D.M. (登録日: 2006-02-26, 公開日: 2006-03-28, 最終更新日: 2024-10-30)

主引用文献

Evdokimov, A.G.,Pokross, M.E.,Egorov, N.S.,Zaraisky, A.G.,Yampolsky, I.V.,Merzlyak, E.M.,Shkoporov, A.N.,Sander, I.,Lukyanov, K.A.,Chudakov, D.M.
Structural basis for the fast maturation of Arthropoda green fluorescent protein.
Embo Rep., 7:1006-1012, 2006

PubMed Abstract: Since the cloning of Aequorea victoria green fluorescent protein (GFP) in 1992, a family of known GFP-like proteins has been growing rapidly. Today, it includes more than a hundred proteins with different spectral characteristics cloned from Cnidaria species. For some of these proteins, crystal structures have been solved, showing diversity in chromophore modifications and conformational states. However, we are still far from a complete understanding of the origin, functions and evolution of the GFP family. Novel proteins of the family were recently cloned from evolutionarily distant marine Copepoda species, phylum Arthropoda, demonstrating an extremely rapid generation of fluorescent signal. Here, we have generated a non-aggregating mutant of Copepoda fluorescent protein and solved its high-resolution crystal structure. It was found that the protein beta-barrel contains a pore, leading to the chromophore. Using site-directed mutagenesis, we showed that this feature is critical for the fast maturation of the chromophore.

PubMed: 16936637
DOI: 10.1038/sj.embor.7400787

実験手法

X-RAY DIFFRACTION (2 Å)