2G6W

Suicide inhibition of a-Oxamine Synthase: Structures of the Covalent Adducts of 8-Amino-7-oxonanoate Synthase with trifluoroalanine

2G6W の概要

• エントリーDOI: 10.2210/pdb2g6w/pdb
• 分子名称: 8-amino-7-oxononanoate synthase, (4-{(E)-[(2,2-DIFLUOROETHYL)IMINO]METHYL}-5-HYDROXY-6-METHYLPYRIDIN-3-YL)METHYL DIHYDROGEN PHOSPHATE (3 entities in total)
• 機能のキーワード: biotin, 8-amino-7-oxonanoate, synthase, plp, fluoroalanine, transferase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 41951.36

構造登録者

Alexeev, D. (登録日: 2006-02-26, 公開日: 2006-04-25, 最終更新日: 2024-11-20)

主引用文献

Alexeev, D.,Baxter, R.L.,Campopiano, D.J.,Kerbarh, O.,Sawyer, L.,Tomczyk, N.,Watt, R.,Webster, S.P.
Suicide inhibition of alpha-oxamine synthases: structures of the covalent adducts of 8-amino-7-oxononanoate synthase with trifluoroalanine.
Org.Biomol.Chem., 4:1209-1212, 2006

PubMed Abstract: The irreversible inhibition of 8-amino-7-oxononanoate synthase by trifluoroalanine involves decarboxylative defluorination of the inhibitor-PLP aldimine followed by attack of the conjugated imine by the amino group of the active site lysine to afford a covalently bound difluorinated intermediate which can subsequently undergo further HF losses and hydrolysis to afford a 2-(pyridoximine phosphate) acetoyl protein adduct.

PubMed: 16557306
DOI: 10.1039/b517922j

実験手法

X-RAY DIFFRACTION (2.14 Å)