2G5Z

Structure of S65G Y66S GFP variant after spontaneous peptide hydrolysis and decarboxylation

Summary for 2G5Z

Related2G16 2G2S 2G3D
DescriptorGreen fluorescent protein, MAGNESIUM ION, ... (4 entities in total)
Functional Keywordschromophore, biosynthesis, peptide hydrolysis, post-translational modification, decarboxylation, luminescent protein
Biological sourceAequorea victoria
Total number of polymer chains2
Total molecular weight26749.31
Authors
Barondeau, D.P.,Kassmann, C.J.,Tainer, J.A.,Getzoff, E.D. (deposition date: 2006-02-23, release date: 2006-04-18, Last modification date: 2011-07-13)
Primary citation
Barondeau, D.P.,Kassmann, C.J.,Tainer, J.A.,Getzoff, E.D.
Understanding GFP Posttranslational Chemistry: Structures of Designed Variants that Achieve Backbone Fragmentation, Hydrolysis, and Decarboxylation.
J.Am.Chem.Soc., 128:4685-4693, 2006
PubMed: 16594705 (PDB entries with the same primary citation)
DOI: 10.1021/ja056635l
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.8 Å)
NMR Information
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.215302.1%0.4%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 2g5z
no rotation
Molmil generated image of 2g5z
rotated about x axis by 90°
Molmil generated image of 2g5z
rotated about y axis by 90°