2G5W

X-ray crystal structure of Arabidopsis thaliana 12-oxophytodienoate reductase isoform 3 (AtOPR3) in complex with 8-iso prostaglandin A1 and its cofactor, flavin mononucleotide.

2G5W の概要

• エントリーDOI: 10.2210/pdb2g5w/pdb
• 関連するPDBエントリー: 1Q45
• 分子名称: 12-oxophytodienoate reductase 3, FLAVIN MONONUCLEOTIDE, (8S,12S)-15S-HYDROXY-9-OXOPROSTA-10Z,13E-DIEN-1-OIC ACID, ... (4 entities in total)
• 機能のキーワード: at2g06050, opr isoform 3, flavoprotein, flavoenzyme, oxidoreductase, xenobiotic reductase, old yellow enzyme, secondary messenger, structural genomics functional follow-up study, protein structure initiative, psi, center for eukaryotic structural genomics, cesg
• 由来する生物種: Arabidopsis thaliana (thale cress)
• 細胞内の位置: Peroxisome : Q9FUP0
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 87067.94

構造登録者

Han, B.W.,Malone, T.E.,Bingman, C.A.,Wesenberg, G.E.,Phillips Jr., G.N.,Fox, B.G.,Center for Eukaryotic Structural Genomics (CESG) (登録日: 2006-02-23, 公開日: 2006-04-04, 最終更新日: 2023-08-30)

主引用文献

Han, B.W.,Malone, T.E.,Kim, D.J.,Bingman, C.A.,Kim, H.J.,Fox, B.G.,Phillips, G.N.
Crystal structure of Arabidopsis thaliana 12-oxophytodienoate reductase isoform 3 in complex with 8-iso prostaglandin A(1).
Proteins, 79:3236-3241, 2011

PubMed Abstract: 12-Oxophytodienoate reductase 3 (OPR3), one of the enzymes involved in the biosynthesis of the plant hormone jasmonic acid (JA), catalyzes the reduction of the cyclopentenone ring of (9,13)-12-oxophytodienoate [(9,13)-OPDA]. However, there has been no structural information about the interaction between OPRs and the physiologically relevant (9,13)-OPDA. Here we report the crystal structure of OPR3 in complex with 8- prostaglandin A1 (8- PGA) which has the same stereochemistry in the cyclopentenone ring as in the physiologically relevant 9,13-OPDA. This structure reveals a new binding mode for substrate that likely contributes to the relaxed stereospecificity observed for AtOPR3.

PubMed: 21915915
DOI: 10.1002/prot.23153

実験手法

X-RAY DIFFRACTION (2.576 Å)