2G3D

Structure of S65G Y66A GFP variant after spontaneous peptide hydrolysis

2G3D の概要

• エントリーDOI: 10.2210/pdb2g3d/pdb
• 関連するPDBエントリー: 2G16 2G2S
• 分子名称: Green fluorescent protein, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: chromophore, biosynthesis, peptide hydrolysis, post-translational modification, luminescent protein
• 由来する生物種: Aequorea victoria; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26761.33

構造登録者

Barondeau, D.P. (登録日: 2006-02-17, 公開日: 2006-04-18, 最終更新日: 2023-08-30)

主引用文献

Barondeau, D.P.,Kassmann, C.J.,Tainer, J.A.,Getzoff, E.D.
Understanding GFP Posttranslational Chemistry: Structures of Designed Variants that Achieve Backbone Fragmentation, Hydrolysis, and Decarboxylation.
J.Am.Chem.Soc., 128:4685-4693, 2006

PubMed Abstract: The green fluorescent protein (GFP) creates a fluorophore out of three sequential amino acids by promoting spontaneous posttranslational modifications. Here, we use high-resolution crystallography to characterize GFP variants that not only undergo peptide backbone cyclization but additional denaturation-induced peptide backbone fragmentation, native peptide hydrolysis, and decarboxylation reactions. Our analyses indicate that architectural features that favor GFP peptide cyclization also drive peptide hydrolysis. These results are relevant for the maturation pathways of GFP homologues, such as the kindling fluorescent protein and the Kaede protein, which use backbone cleavage to red-shift the spectral properties of their chromophores. We further propose a photochemical mechanism for the decarboxylation reaction, supporting a role for the GFP protein environment in facilitating radical formation and one-electron chemistry, which may be important in activating oxygen for the oxidation step of chromophore biosynthesis. Together, our results characterize GFP posttranslational modification chemistry with implications for the energetic landscape of backbone cyclization and subsequent reactions, and for the rational design of predetermined spontaneous backbone cyclization and cleavage reactions.

PubMed: 16594705
DOI: 10.1021/ja056635l

実験手法

X-RAY DIFFRACTION (1.35 Å)