2G37

Structure of Thermus thermophilus L-proline dehydrogenase

2G37 の概要

• エントリーDOI: 10.2210/pdb2g37/pdb
• 分子名称: proline dehydrogenase/delta-1-pyrroline-5-carboxylate dehydrogenase, FLAVIN-ADENINE DINUCLEOTIDE, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (4 entities in total)
• 機能のキーワード: beta8-alpha8-barrel, flavoenzyme, oxidoreductase
• 由来する生物種: Thermus thermophilus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 78135.50

構造登録者

Tanner, J.J.,White, T.A. (登録日: 2006-02-17, 公開日: 2007-02-27, 最終更新日: 2024-02-14)

主引用文献

White, T.A.,Krishnan, N.,Becker, D.F.,Tanner, J.J.
Structure and Kinetics of Monofunctional Proline Dehydrogenase from Thermus thermophilus.
J.Biol.Chem., 282:14316-14327, 2007

PubMed Abstract: Proline dehydrogenase (PRODH) and Delta(1)-pyrroline-5-carboxylate dehydrogenase (P5CDH) catalyze the two-step oxidation of proline to glutamate. They are distinct monofunctional enzymes in all eukaryotes and some bacteria but are fused into bifunctional enzymes known as proline utilization A (PutA) in other bacteria. Here we report the first structure and biochemical data for a monofunctional PRODH. The 2.0-A resolution structure of Thermus thermophilus PRODH reveals a distorted (betaalpha)(8) barrel catalytic core domain and a hydrophobic alpha-helical domain located above the carboxyl-terminal ends of the strands of the barrel. Although the catalytic core is similar to that of the PutA PRODH domain, the FAD conformation of T. thermophilus PRODH is remarkably different and likely reflects unique requirements for membrane association and communication with P5CDH. Also, the FAD of T. thermophilus PRODH is highly solvent-exposed compared with PutA due to a 4-A shift of helix 8. Structure-based sequence analysis of the PutA/PRODH family led us to identify nine conserved motifs involved in cofactor and substrate recognition. Biochemical studies show that the midpoint potential of the FAD is -75 mV and the kinetic parameters for proline are K(m) = 27 mm and k(cat) = 13 s(-1). 3,4-Dehydro-l-proline was found to be an efficient substrate, and l-tetrahydro-2-furoic acid is a competitive inhibitor (K(I) = 1.0 mm). Finally, we demonstrate that T. thermophilus PRODH reacts with O(2) producing superoxide. This is significant because superoxide production underlies the role of human PRODH in p53-mediated apoptosis, implying commonalities between eukaryotic and bacterial monofunctional PRODHs.

PubMed: 17344208
DOI: 10.1074/jbc.M700912200

実験手法

X-RAY DIFFRACTION (2 Å)