2G2X

X-Ray Crystal Structure Protein Q88CH6 from Pseudomonas putida. Northeast Structural Genomics Consortium Target PpR72.

Summary for 2G2X

• Entry DOI: 10.2210/pdb2g2x/pdb
• Related: 1ZCE 2AR1 2EVE
• Descriptor: hypothetical protein PP5205, SULFATE ION (3 entities in total)
• Functional Keywords: ppr72, nesg, structural genomics, psi, protein structure initiative, northeast structural genomics consortium, unknown function
• Biological source: Pseudomonas putida
• Total number of polymer chains: 3
• Total formula weight: 54530.72

Authors

Kuzin, A.P.,Chen, Y.,Abashidze, M.,Acton, T.,Conover, K.,Janjua, H.,Ma, L.-C.,Ho, C.K.,Cunningham, K.,Montelione, G.,Hunt, J.F.,Tong, L.,Northeast Structural Genomics Consortium (NESG) (deposition date: 2006-02-16, release date: 2006-03-14, Last modification date: 2023-11-15)

Primary citation

Bertonati, C.,Punta, M.,Fischer, M.,Yachdav, G.,Forouhar, F.,Zhou, W.,Kuzin, A.P.,Seetharaman, J.,Abashidze, M.,Ramelot, T.A.,Kennedy, M.A.,Cort, J.R.,Belachew, A.,Hunt, J.F.,Tong, L.,Montelione, G.T.,Rost, B.
Structural genomics reveals EVE as a new ASCH/PUA-related domain.
Proteins, 75:760-773, 2009

PubMed Abstract: We report on several proteins recently solved by structural genomics consortia, in particular by the Northeast Structural Genomics consortium (NESG). The proteins considered in this study differ substantially in their sequences but they share a similar structural core, characterized by a pseudobarrel five-stranded beta sheet. This core corresponds to the PUA domain-like architecture in the SCOP database. By connecting sequence information with structural knowledge, we characterize a new subgroup of these proteins that we propose to be distinctly different from previously described PUA domain-like domains such as PUA proper or ASCH. We refer to these newly defined domains as EVE. Although EVE may have retained the ability of PUA domains to bind RNA, the available experimental and computational data suggests that both the details of its molecular function and its cellular function differ from those of other PUA domain-like domains. This study of EVE and its relatives illustrates how the combination of structure and genomics creates new insights by connecting a cornucopia of structures that map to the same evolutionary potential. Primary sequence information alone would have not been sufficient to reveal these evolutionary links.

PubMed: 19191354
DOI: 10.1002/prot.22287

Experimental method

X-RAY DIFFRACTION (2.3 Å)