2G2X

X-Ray Crystal Structure Protein Q88CH6 from Pseudomonas putida. Northeast Structural Genomics Consortium Target PpR72.

2G2X の概要

• エントリーDOI: 10.2210/pdb2g2x/pdb
• 関連するPDBエントリー: 1ZCE 2AR1 2EVE
• 分子名称: hypothetical protein PP5205, SULFATE ION (3 entities in total)
• 機能のキーワード: ppr72, nesg, structural genomics, psi, protein structure initiative, northeast structural genomics consortium, unknown function
• 由来する生物種: Pseudomonas putida
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 54530.72

構造登録者

Kuzin, A.P.,Chen, Y.,Abashidze, M.,Acton, T.,Conover, K.,Janjua, H.,Ma, L.-C.,Ho, C.K.,Cunningham, K.,Montelione, G.,Hunt, J.F.,Tong, L.,Northeast Structural Genomics Consortium (NESG) (登録日: 2006-02-16, 公開日: 2006-03-14, 最終更新日: 2024-11-20)

主引用文献

Bertonati, C.,Punta, M.,Fischer, M.,Yachdav, G.,Forouhar, F.,Zhou, W.,Kuzin, A.P.,Seetharaman, J.,Abashidze, M.,Ramelot, T.A.,Kennedy, M.A.,Cort, J.R.,Belachew, A.,Hunt, J.F.,Tong, L.,Montelione, G.T.,Rost, B.
Structural genomics reveals EVE as a new ASCH/PUA-related domain.
Proteins, 75:760-773, 2009

PubMed Abstract: We report on several proteins recently solved by structural genomics consortia, in particular by the Northeast Structural Genomics consortium (NESG). The proteins considered in this study differ substantially in their sequences but they share a similar structural core, characterized by a pseudobarrel five-stranded beta sheet. This core corresponds to the PUA domain-like architecture in the SCOP database. By connecting sequence information with structural knowledge, we characterize a new subgroup of these proteins that we propose to be distinctly different from previously described PUA domain-like domains such as PUA proper or ASCH. We refer to these newly defined domains as EVE. Although EVE may have retained the ability of PUA domains to bind RNA, the available experimental and computational data suggests that both the details of its molecular function and its cellular function differ from those of other PUA domain-like domains. This study of EVE and its relatives illustrates how the combination of structure and genomics creates new insights by connecting a cornucopia of structures that map to the same evolutionary potential. Primary sequence information alone would have not been sufficient to reveal these evolutionary links.

PubMed: 19191354
DOI: 10.1002/prot.22287

実験手法

X-RAY DIFFRACTION (2.3 Å)