2G2K

NMR structure of an N-terminal fragment of the eukaryotic initiation factor 5 (eIF5)

2G2K の概要

• エントリーDOI: 10.2210/pdb2g2k/pdb
• 分子名称: Eukaryotic translation initiation factor 5 (1 entity in total)
• 機能のキーワード: eif5, eif125 fold, translation
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19274.21

構造登録者

Conte, M.R.,Kelly, G.,Babon, J.,Sanfelice, D.,Smerdon, S.J.,Proud, C.G. (登録日: 2006-02-16, 公開日: 2006-06-13, 最終更新日: 2024-05-29)

主引用文献

Conte, M.R.,Kelly, G.,Babon, J.,Sanfelice, D.,Youell, J.,Smerdon, S.J.,Proud, C.G.
Structure of the eukaryotic initiation factor (eIF) 5 reveals a fold common to several translation factors
Biochemistry, 45:4550-4558, 2006

PubMed Abstract: Eukaryotic initiation factor 5 (eIF5) plays multiple roles in translation initiation. Its N-terminal domain functions as a GTPase-activator protein (GAP) for GTP bound to eIF2, while its C-terminal region nucleates the interactions between multiple translation factors, including eIF1, which acts to inhibit GTP hydrolysis or P(i) release, and the beta subunit of eIF2. These proteins and the events in which they participate are critical for the accurate recognition of the correct start codon during translation initiation. Here, we report the three-dimensional solution structure of the N-terminal domain of human eIF5, comprising two subdomains, both reminiscent of nucleic-acid-binding modules. The N-terminal subdomain contains the "arginine finger" motif that is essential for GAP function but which, unusually, resides in a partially disordered region of the molecule. This implies that a conformational reordering of this portion of eIF5 is likely to occur upon formation of a competent complex for GTP hydrolysis, following the appropriate activation signal. Interestingly, the N-terminal subdomain of eIF5 reveals an alpha/beta fold structurally similar to both the archaeal orthologue of the beta subunit of eIF2 and, unexpectedly, to eIF1. These results reveal a novel protein fold common to several factors involved in related steps of translation initiation. The implications of these observations are discussed in terms of the mechanism of translation initiation.

PubMed: 16584190
DOI: 10.1021/bi052387u

実験手法

SOLUTION NMR