2G1W

NMR structure of the Aquifex aeolicus tmRNA pseudoknot PK1

Summary for 2G1W

• Entry DOI: 10.2210/pdb2g1w/pdb
• Descriptor: 5'-R(*GP*GP*GP*GP*UP*GP*GP*CP*UP*CP*CP*CP*CP*UP*AP*AP*CP*AP*GP*CP*CP*G)-3' (1 entity in total)
• Functional Keywords: tmrna, pk1, pseudoknot, trans-translation, rna
• Total number of polymer chains: 1
• Total formula weight: 7064.26

Authors

Nonin-Lecomte, S.,Dardel, F. (deposition date: 2006-02-15, release date: 2006-04-11, Last modification date: 2024-05-29)

Primary citation

Nonin-Lecomte, S.,Felden, B.,Dardel, F.
NMR structure of the Aquifex aeolicus tmRNA pseudoknot PK1: new insights into the recoding event of the ribosomal trans-translation.
Nucleic Acids Res., 34:1847-1853, 2006

PubMed Abstract: The transfer-messenger RNA (tmRNA) pseudoknot PK1 is essential for bacterial trans-translation, a ribosomal rescue mechanism. We report the solution structure of PK1 from Aquifex aeolicus, which despite an unprecedented small number of nucleotides and thus an unprecented compact size, displays a very high thermal stability. Several unusual structural features account for these properties and indicate that PK1 belongs to the class of ribosomal frameshift pseudoknots. This suggests a similarity between the mechanism of programmed ribosomal frameshifting and trans-translation.

PubMed: 16595798
DOI: 10.1093/nar/gkl111

Experimental method

SOLUTION NMR