2G1G
Solution structure of the anticodon loop of S. Pombe tRNAi including the naturally occurring N6-threonyl adenine
Summary for 2G1G
| Entry DOI | 10.2210/pdb2g1g/pdb |
| Related | 1FEQ |
| Descriptor | 5'-R(*AP*CP*GP*GP*GP*CP*UP*CP*AP*UP*(T6A)P*AP*CP*CP*CP*GP*U)-3' (1 entity in total) |
| Functional Keywords | t-rnai anticodonloop t6a, rna |
| Total number of polymer chains | 1 |
| Total formula weight | 5547.39 |
| Authors | Lescrinier, E. (deposition date: 2006-02-14, release date: 2006-06-20, Last modification date: 2024-05-29) |
| Primary citation | Lescrinier, E.,Nauwelaerts, K.,Zanier, K.,Poesen, K.,Sattler, M.,Herdewijn, P. The naturally occurring N6-threonyl adenine in anticodon loop of Schizosaccharomyces pombe tRNAi causes formation of a unique U-turn motif Nucleic Acids Res., 34:2878-2886, 2006 Cited by PubMed Abstract: Modified nucleosides play an important role in structure and function of tRNA. We have determined the solution structure of the anticodon stem-loop (ASL) of initiator tRNA of Schizosaccharomyces pombe. The incorporation of N6-threonylcarbamoyladenosine at the position 3' to the anticodon triplet (t6A37) results in the formation of a U-turn motif and enhances stacking interactions within the loop and stem regions (i.e. between A35 and t6A37) by bulging out U36. This conformation was not observed in a crystal structure of tRNAi including the same modification in its anticodon loop, nor in the solution structure of the unmodified ASL. A t6A modification also occurs in the well studied anti-stem-loop of lys-tRNA(UUU). A comparison of this stem-loop with our structure demonstrates different effects of the modification depending on the loop sequence. PubMed: 16738127DOI: 10.1093/nar/gkl081 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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