2G1G

Solution structure of the anticodon loop of S. Pombe tRNAi including the naturally occurring N6-threonyl adenine

2G1G の概要

• エントリーDOI: 10.2210/pdb2g1g/pdb
• 関連するPDBエントリー: 1FEQ
• 分子名称: 5'-R(*AP*CP*GP*GP*GP*CP*UP*CP*AP*UP*(T6A)P*AP*CP*CP*CP*GP*U)-3' (1 entity in total)
• 機能のキーワード: t-rnai anticodonloop t6a, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5547.39

構造登録者

Lescrinier, E. (登録日: 2006-02-14, 公開日: 2006-06-20, 最終更新日: 2024-05-29)

主引用文献

Lescrinier, E.,Nauwelaerts, K.,Zanier, K.,Poesen, K.,Sattler, M.,Herdewijn, P.
The naturally occurring N6-threonyl adenine in anticodon loop of Schizosaccharomyces pombe tRNAi causes formation of a unique U-turn motif
Nucleic Acids Res., 34:2878-2886, 2006

PubMed Abstract: Modified nucleosides play an important role in structure and function of tRNA. We have determined the solution structure of the anticodon stem-loop (ASL) of initiator tRNA of Schizosaccharomyces pombe. The incorporation of N6-threonylcarbamoyladenosine at the position 3' to the anticodon triplet (t6A37) results in the formation of a U-turn motif and enhances stacking interactions within the loop and stem regions (i.e. between A35 and t6A37) by bulging out U36. This conformation was not observed in a crystal structure of tRNAi including the same modification in its anticodon loop, nor in the solution structure of the unmodified ASL. A t6A modification also occurs in the well studied anti-stem-loop of lys-tRNA(UUU). A comparison of this stem-loop with our structure demonstrates different effects of the modification depending on the loop sequence.

PubMed: 16738127
DOI: 10.1093/nar/gkl081

実験手法

SOLUTION NMR