2FZ0

Identification of yeast R-SNARE Nyv1p as a novel longin domain protein

2FZ0 の概要

• エントリーDOI: 10.2210/pdb2fz0/pdb
• 分子名称: v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane; Nyv1p (1 entity in total)
• 機能のキーワード: snare protein, longin domain, membrane protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Vacuole membrane; Single-pass type IV membrane protein: Q12255
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17014.38

構造登録者

Wen, W.,Zhang, M. (登録日: 2006-02-08, 公開日: 2006-03-07, 最終更新日: 2024-05-29)

主引用文献

Wen, W.,Chen, L.,Wu, H.,Sun, X.,Zhang, M.,Banfield, D.K.
Identification of the Yeast R-SNARE Nyv1p as a Novel Longin Domain-containing Protein
Mol.Cell.Biol., 17:4282-4299, 2006

PubMed Abstract: Using nuclear magnetic resonance spectroscopy, we establish that the N-terminal domain of the yeast vacuolar R-SNARE Nyv1p adopts a longin-like fold similar to those of Sec22b and Ykt6p. Nyv1p is sorted to the limiting membrane of the vacuole via the adaptor protein (AP)3 adaptin pathway, and we show that its longin domain is sufficient to direct transport to this location. In contrast, we found that the longin domains of Sec22p and Ykt6p were not sufficient to direct their localization. A YXX phi-like adaptin-dependent sorting signal (Y31GTI34) unique to the longin domain of Nyv1p mediates interactions with the AP3 complex in vivo and in vitro. We show that amino acid substitutions to Y31GTI34 (Y31Q;I34Q) resulted in mislocalization of Nyv1p as well as reduced binding of the mutant protein to the AP3 complex. Although the sorting of Nyv1p to the limiting membrane of the vacuole is dependent upon the Y31GTI34 motif, and Y31 in particular, our findings with structure-based amino acid substitutions in the mu chain (Apm3p) of yeast AP3 suggest a mechanistically distinct role for this subunit in the recognition of YXX phi-like sorting signals.

PubMed: 16855025
DOI: 10.1091/mbc.E06-02-0128

実験手法

SOLUTION NMR