2FX0

Crystal Structure of HlyIIR, a Hemolysin II transcriptional Regulator

2FX0 の概要

• エントリーDOI: 10.2210/pdb2fx0/pdb
• 分子名称: hemolysin II regulatory protein (2 entities in total)
• 機能のキーワード: transcriptional regulator, transcription
• 由来する生物種: Bacillus cereus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23575.23

構造登録者

Kovalevskiy, O.V.,Lebedev, A.A.,Solonin, A.S.,Antson, A.A. (登録日: 2006-02-03, 公開日: 2006-02-21, 最終更新日: 2024-11-20)

主引用文献

Kovalevskiy, O.V.,Lebedev, A.A.,Surin, A.K.,Solonin, A.S.,Antson, A.A.
Crystal Structure of Bacillus cereus HlyIIR, a Transcriptional Regulator of the Gene for Pore-forming Toxin Hemolysin II.
J.Mol.Biol., 365:825-834, 2007

PubMed Abstract: Production of Bacillus cereus and Bacillus anthracis toxins is controlled by a number of transcriptional regulators. Here we report the crystal structure of B. cereus HlyIIR, a regulator of the gene encoding the pore-forming toxin hemolysin II. We show that HlyIIR forms a tight dimer with a fold and overall architecture similar to the TetR family of repressors. A remarkable feature of the structure is a large internal cavity with a volume of 550 A(3) suggesting that the activity of HlyIIR is modulated by binding of a ligand, which triggers the toxin production. Virtual ligand library screening shows that this pocket can accommodate compounds with molecular masses of up to 400-500 Da. Based on structural data and previous biochemical evidence, we propose a model for HlyIIR interaction with the DNA.

PubMed: 17097673
DOI: 10.1016/j.jmb.2006.10.074

実験手法

X-RAY DIFFRACTION (2.4 Å)