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2FVQ

A Structural Study of the CA Dinucleotide Step in the Integrase Processing Site of Moloney Murine Leukemia Virus

Summary for 2FVQ
Entry DOI10.2210/pdb2fvq/pdb
Related2FVP 2FVR 2FVS
Descriptor5'-D(*CP*TP*TP*TP*CP*AP*TP*TP*AP*AP*TP*GP*AP*AP*AP*G)-3', reverse transcriptase (3 entities in total)
Functional Keywordsltr, mmlv, transferase-dna complex, transferase/dna
Biological sourceMoloney murine leukemia virus
Cellular locationGag-Pol polyprotein: Host cell membrane; Lipid-anchor (Potential). Matrix protein p15: Virion (Potential). Capsid protein p30: Virion (Potential). Nucleocapsid protein p10: Virion (Potential): P03355
Total number of polymer chains2
Total formula weight33830.50
Authors
Montano, S.P.,Cote, M.L.,Roth, M.J.,Georgiadis, M.M. (deposition date: 2006-01-31, release date: 2006-12-12, Last modification date: 2023-08-30)
Primary citationMontano, S.P.,Cote, M.L.,Roth, M.J.,Georgiadis, M.M.
Crystal structures of oligonucleotides including the integrase processing site of the Moloney murine leukemia virus.
Nucleic Acids Res., 34:5353-5360, 2006
Cited by
PubMed Abstract: In the first step of retroviral integration, integrase cleaves the linear viral DNA within its long terminal repeat (LTR) immediately 3' to the CA dinucleotide step, resulting in a reactive 3' OH on one strand and a 5' two base overhang on the complementary strand. In order to investigate the structural properties of the 3' end processing site within the Moloney murine leukemia virus (MMLV) LTR d(TCTTTCATT), a host-guest crystallographic method was employed to determine the structures of four self-complementary 16 bp oligonucleotides including LTR sequences (underlined), d(TTTCATTGCAATGAAA), d(CTTTCATTAATGAAAG), d(TCTTTCATATGAAAGA) and d(CACAATGATCATTGTG), the guests, complexed with the N-terminal fragment of MMLV reverse transcriptase, the host. The structures of the LTR-containing oligonucleotides were compared to those of non-LTR oligonucleotides crystallized in the same lattice. Properties unique to the CA dinucleotide step within the LTR sequence, independent of its position from the end of the duplex, include a positive roll angle and negative slide value. This propensity for the CA dinucleotide step within the MMLV LTR sequence to adopt only positive roll angles is likely influenced by the more rigid, invariable 3' and 5' flanking TT dinucleotide steps and may be important for specific recognition and/or cleavage by the MMLV integrase.
PubMed: 17003051
DOI: 10.1093/nar/gkl693
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.3 Å)
Structure validation

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數據於2024-11-06公開中

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