2FUL

Crystal Structure of the C-terminal Domain of S. cerevisiae eIF5

2FUL の概要

• エントリーDOI: 10.2210/pdb2ful/pdb
• 分子名称: Eukaryotic translation initiation factor 5, SULFATE ION (3 entities in total)
• 機能のキーワード: atypical heat motif, translation
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 124398.63

構造登録者

Wei, Z.,Xue, Y.,Xu, H.,Gong, W. (登録日: 2006-01-27, 公開日: 2006-05-23, 最終更新日: 2024-03-13)

主引用文献

Wei, Z.,Xue, Y.,Xu, H.,Gong, W.
Crystal Structure of the C-terminal Domain of S.cerevisiae eIF5
J.Mol.Biol., 359:1-9, 2006

PubMed Abstract: eIF5, a GTPase-activating protein (GAP) specific for eIF2, plays a critical role in pre-initiation complex assembly and correct AUG selection during eukaryotic translation initiation. eIF5 is involved in the formation of the multifactor complex (MFC), an important intermediate of the 43S pre-initiation complex. The C-terminal domain (CTD) of eIF5 functions as the structural core in the MFC assembly. Here we report the 1.5A crystal structure of eIF5-CTD, confirming that eIF5-CTD contains an atypical HEAT motif. In addition, analyzing the electrostatic potential and the distribution of conserved residues on the protein surface, we confirm and suggest some potential regions of interactions between eIF5-CTD and other eIFs. The structure of eIF5-CTD provides useful information in understanding the mechanism of the MFC assembly.

PubMed: 16616930
DOI: 10.1016/j.jmb.2006.03.037

実験手法

X-RAY DIFFRACTION (1.5 Å)