2FUB

Crystal structure of urate oxidase at 140 MPa

2FUB の概要

• エントリーDOI: 10.2210/pdb2fub/pdb
• 関連するPDBエントリー: 1R51
• 分子名称: Uricase, 8-AZAXANTHINE, CYSTEINE, ... (4 entities in total)
• 機能のキーワード: tetramer, high pressure, oxidoreductase, hpmx
• 由来する生物種: Aspergillus flavus
• 細胞内の位置: Peroxisome: Q00511
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34431.81

構造登録者

Colloc'h, N.,Girard, E.,Fourme, R. (登録日: 2006-01-26, 公開日: 2006-02-14, 最終更新日: 2024-10-30)

主引用文献

Colloc'h, N.,Girard, E.,Dhaussy, A.-C.,Kahn, R.,Ascone, I.,Mezouar, M.,Fourme, R.
High pressure macromolecular crystallography: The 140-MPa crystal structure at 2.3 A resolution of urate oxidase, a 135-kDa tetrameric assembly
Biochim.Biophys.Acta, 1764:391-397, 2006

PubMed Abstract: We report the three-dimensional structure determined by high-pressure macromolecular crystallography (HPMX) of a 135-kDa homo-tetrameric enzyme, urate oxidase from Aspergillus flavus complexed with its potent inhibitor 8-azaxanthin. Urate oxidase crystals are quite sensitive to pressure, as three-dimensional order is lost at about 180 MPa. A highly complete 2.3 A resolution data set was collected at 140 MPa, close to the critical pressure. Crystal structures at atmospheric pressure and at high pressure were refined in the orthorhombic space group I222 with final crystallographic R factors 14.1% and 16.1%, respectively. The effect of pressure on temperature factors, ordered water molecules, hydrogen bond lengths, contacts, buried surface areas as well as cavity volume was investigated. Results suggest that the onset of disruption of the tetrameric assembly by pressure has been captured in the crystalline state.

PubMed: 16478683
DOI: 10.1016/j.bbapap.2006.01.006

実験手法

X-RAY DIFFRACTION (2.3 Å)