2FTB

Crystal structure of axolotl (Ambystoma mexicanum) liver bile acid-binding protein bound to oleic acid

2FTB の概要

• エントリーDOI: 10.2210/pdb2ftb/pdb
• 関連するPDBエントリー: 2FT9
• 分子名称: Fatty acid-binding protein 2, liver, OLEIC ACID (3 entities in total)
• 機能のキーワード: liver bile acid-binding protein, liver basic fatty acid-binding protein, axolotl, oleic acid, babp, lb-fabp, lipid binding protein
• 由来する生物種: Ambystoma mexicanum (axolotl)
• 細胞内の位置: Cytoplasm: P81400
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14041.04

構造登録者

Capaldi, S.,Guariento, M.,Perduca, M.,Di Pietro, S.M.,Santome, J.A.,Monaco, H.L. (登録日: 2006-01-24, 公開日: 2006-04-11, 最終更新日: 2023-10-25)

主引用文献

Capaldi, S.,Guariento, M.,Perduca, M.,Di Pietro, S.M.,Santome, J.A.,Monaco, H.L.
Crystal structure of axolotl (Ambystoma mexicanum) liver bile acid-binding protein bound to cholic and oleic acid
Proteins, 64:79-88, 2006

PubMed Abstract: The family of the liver bile acid-binding proteins (L-BABPs), formerly called liver basic fatty acid-binding proteins (Lb-FABPs) shares fold and sequence similarity with the paralogous liver fatty acid-binding proteins (L-FABPs) but has a different stoichiometry and specificity of ligand binding. This article describes the first X-ray structure of a member of the L-BABP family, axolotl (Ambystoma mexicanum) L-BABP, bound to two different ligands: cholic and oleic acid. The protein binds one molecule of oleic acid in a position that is significantly different from that of either of the two molecules that bind to rat liver FABP. The stoichiometry of binding of cholate is of two ligands per protein molecule, as observed in chicken L-BABP. The cholate molecule that binds buried most deeply into the internal cavity overlaps well with the analogous bound to chicken L-BABP, whereas the second molecule, which interacts with the first only through hydrophobic contacts, is more external and exposed to the solvent.

PubMed: 16555310
DOI: 10.1002/prot.20961

実験手法

X-RAY DIFFRACTION (2 Å)