2FR4

Structure of Fab DNA-1 complexed with a stem-loop DNA ligand

2FR4 の概要

• エントリーDOI: 10.2210/pdb2fr4/pdb
• 関連するPDBエントリー: 1I8M 1XF2 1XF3 1XF4
• 分子名称: 5'-D(*CP*TP*GP*CP*CP*TP*TP*CP*AP*G)-3', antibody light chain FAB, antibody heavy chain FAB, ... (5 entities in total)
• 機能のキーワード: antibody, fab, immunoglobulin, anti-dna, anti-ssdna, autoantibody, stem-loop dna, immune system-dna complex, immune system/dna
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 103382.35

構造登録者

Tanner, J.J.,Ou, Z. (登録日: 2006-01-18, 公開日: 2007-01-09, 最終更新日: 2024-11-13)

主引用文献

Ou, Z.,Bottoms, C.A.,Henzl, M.T.,Tanner, J.J.
Impact of DNA hairpin folding energetics on antibody-ssDNA association.
J.Mol.Biol., 374:1029-1040, 2007

PubMed Abstract: Deposition of anti-DNA antibodies in the kidney contributes to the pathogenesis of the autoimmune disease, systemic lupus erythematosus. Antibodies that bind to hairpin-forming DNA ligands may be particularly prone to deposition. Here we report the first structure of a Fab complexed with hairpin-forming DNA. The ligand used for co-crystallization is 5'-d [CTG(CCTT)CAG]-3', which has a predicted hairpin structure consisting of a four-nucleotide loop (CCTT) and a stem of three base-pairs. The 1.95 A resolution crystal structure of Fab DNA-1 complexed with this ligand shows that the conformation of the bound ligand differs radically from the predicted hairpin conformation. The three base-pairs in the stem are absent in the bound form. The protein binds to the last six nucleotides at the 3' end of the ligand. These nucleotides form a loop (TTCA) closed by a G:C base-pair in the bound state. Stacking of aromatic side-chains against DNA bases is the dominant interaction in the complex. Interactions with the DNA backbone are conspicuously absent. Thermodynamics of binding are examined using isothermal titration calorimetry. The apparent dissociation constant is 4 microM, and binding is enthalpically favorable and entropically unfavorable. Increasing the number of base-pairs in the DNA stem from three to six decreases binding affinity. These data suggest a conformational selection binding mechanism in which the Fab binds preferentially to the unstructured state of the ligand. In this interpretation, the ligand binding and ligand folding equilibria are coupled, with lower hairpin stability leading to greater effective binding affinity. Thus, pre-organization of the DNA loop into the preferred binding conformation does not play a major role in complexation. Rather, it is argued that the stem of the hairpin serves to reduce the degrees of freedom in the free DNA ligand, thereby limiting the entropic cost attendant to complexation with the Fab.

PubMed: 18028946
DOI: 10.1016/j.jmb.2007.09.084

実験手法

X-RAY DIFFRACTION (1.95 Å)