2FQN

Crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site

Summary for 2FQN

• Entry DOI: 10.2210/pdb2fqn/pdb
• Descriptor: 5'-R(*UP*UP*GP*CP*GP*UP*CP*GP*CP*UP*CP*CP*GP*GP*AP*AP*AP*AP*GP*UP*CP*GP*C)-3', MAGNESIUM ION, COBALT HEXAMMINE(III), ... (4 entities in total)
• Functional Keywords: rna duplex, rrna, decoding site, a site, homo sapiens cytoplasm, rna
• Total number of polymer chains: 2
• Total formula weight: 15242.78

Authors

Kondo, J.,Urzhumtsev, A.,Westhof, E. (deposition date: 2006-01-18, release date: 2006-02-14, Last modification date: 2024-02-14)

Primary citation

Kondo, J.,Urzhumtsev, A.,Westhof, E.
Two conformational states in the crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site.
Nucleic Acids Res., 34:676-685, 2006

PubMed Abstract: The decoding A site of the small ribosomal subunit is an RNA molecular switch, which monitors codon-anticodon interactions to guarantee translation fidelity. We have solved the crystal structure of an RNA fragment containing two Homo sapiens cytoplasmic A sites. Each of the two A sites presents a different conformational state. In one state, adenines A1492 and A1493 are fully bulged-out with C1409 forming a wobble-like pair to A1491. In the second state, adenines A1492 and A1493 form non-Watson-Crick pairs with C1409 and G1408, respectively while A1491 bulges out. The first state of the eukaryotic A site is, thus, basically the same as in the bacterial A site with bulging A1492 and A1493. It is the state used for recognition of the codon/anticodon complex. On the contrary, the second state of the H.sapiens cytoplasmic A site is drastically different from any of those observed for the bacterial A site without bulging A1492 and A1493.

PubMed: 16452297
DOI: 10.1093/nar/gkj467

Experimental method

X-RAY DIFFRACTION (2.3 Å)