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2FQE

Crystal Structures of E. coli Laccase CueO under different copper binding situations

2FQE の概要
エントリーDOI10.2210/pdb2fqe/pdb
関連するPDBエントリー2FQD 2FQF 2FQG
分子名称Blue copper oxidase cueO, COPPER (II) ION, SODIUM ION, ... (6 entities in total)
機能のキーワードazurin-like domain, oxidoreductase
由来する生物種Escherichia coli
細胞内の位置Periplasm: P36649
タンパク質・核酸の鎖数1
化学式量合計53968.58
構造登録者
Li, X.,Wei, Z.,Zhang, M.,Teng, M.,Gong, W. (登録日: 2006-01-18, 公開日: 2007-01-30, 最終更新日: 2024-03-13)
主引用文献Li, X.,Wei, Z.,Zhang, M.,Peng, X.,Yu, G.,Teng, M.,Gong, W.
Crystal structures of E. coli laccase CueO at different copper concentrations.
Biochem.Biophys.Res.Commun., 354:21-26, 2007
Cited by
PubMed Abstract: CueO protein is a hypothetical bacterial laccase and a good laccase candidate for large scale industrial application. Four CueO crystal structures were determined at different copper concentrations. Low copper occupancy in apo-CueO and slow copper reconstitution process in CueO with exogenous copper were demonstrated. These observations well explain the copper dependence of CueO oxidase activity. Structural comparison between CueO and other three fungal laccase proteins indicates that Glu106 in CueO constitutes the primary counter-work for reconstitution of the trinuclear copper site. Mutation of Glu106 to a Phe enhanced CueO oxidation activity and supported this hypothesis. In addition, an extra alpha-helix from Leu351 to Gly378 covers substrate biding pocket of CueO and might compromises the electron transfer from substrate to type I copper.
PubMed: 17217912
DOI: 10.1016/j.bbrc.2006.12.116
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (1.92 Å)
構造検証レポート
Validation report summary of 2fqe
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-09に公開中

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