2FPK

RadA recombinase in complex with ADP

「1Z4B」から置き換えられました

2FPK の概要

• エントリーDOI: 10.2210/pdb2fpk/pdb
• 関連するPDBエントリー: 2FPL 2FPM
• 分子名称: DNA repair and recombination protein radA, MAGNESIUM ION, POTASSIUM ION, ... (5 entities in total)
• 機能のキーワード: atpase, protein-atp complex, rada-adp complex, co-factors, potassium-dependence, recombination
• 由来する生物種: Methanococcus voltae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35713.84

構造登録者

Wu, Y.,Qian, X.,He, Y.,Moya, I.A.,Luo, Y. (登録日: 2006-01-16, 公開日: 2006-01-31, 最終更新日: 2023-08-30)

主引用文献

Qian, X.,Wu, Y.,He, Y.,Moya, I.A.,Luo, Y.
Crystal Structure of Methanococcus Voltae Rada in Complex with Adp: hydrolysis-induced conformational change
Biochemistry, 44:13753-13761, 2005

PubMed Abstract: Members of a superfamily of RecA-like recombinases facilitate a central strand exchange reaction in the DNA repair process. Archaeal RadA and Rad51 and eukaryal Rad51 and meiosis-specific DMC1 form a closely related group of recombinases distinct from bacterial RecA. Nevertheless, all such recombinases share a conserved core domain which carries the ATPase site and putative DNA-binding sites. Here we present the crystal structure of an archaeal RadA from Methanococcus voltae (MvRadA) in complex with ADP and Mg2+ at 2.1 A resolution. The crystallized RadA-ADP filament has an extended helical pitch similar to those of previously determined structures in the presence of nonhydrolyzable ATP analogue AMP-PNP. Structural comparison reveals two recurrent conformations with an extensive allosteric effect spanning the ATPase site and the putative DNA-binding L2 region. Varied conformations of the L2 region also imply a dynamic nature of recombinase-bound DNA.

PubMed: 16229465
DOI: 10.1021/bi051222i

実験手法

X-RAY DIFFRACTION (2.1 Å)