2FNF

C1 domain of Nore1

2FNF の概要

• エントリーDOI: 10.2210/pdb2fnf/pdb
• 関連するPDBエントリー: 1RFH
• 分子名称: putative Ras Effector Nore1, ZINC ION (2 entities in total)
• 機能のキーワード: zinc; signal transduction; apoptosis; cysteine rich domain, apoptosis
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8400.34

構造登録者

Harjes, E.,Harjes, S.,Wohlgemuth, S.,Krieger, E.,Herrmann, C.,Muller, K.H.,Bayer, P. (登録日: 2006-01-11, 公開日: 2006-02-07, 最終更新日: 2024-05-29)

主引用文献

Harjes, E.,Harjes, S.,Wohlgemuth, S.,Muller, K.H.,Krieger, E.,Herrmann, C.,Bayer, P.
GTP-Ras disrupts the intramolecular complex of C1 and RA domains of Nore1.
Structure, 14:881-888, 2006

PubMed Abstract: The novel Ras effector mNore1, capable of inducing apoptosis, is a multidomain protein. It comprises a C1 domain homologous to PKC and an RA domain similar to the Ras effectors AF-6 and RalGDS. Here, we determine the affinity of these two domains to the active forms of Ras and Rap1 using isothermal calorimetric titration. The interaction of Ras/Rap1-GTP with the RA domain of mNore1 is weakened significantly by direct binding of the C1 domain to the RA domain. In order to analyze this observation in atomic detail, we solved the C1 solution structure by NMR. By determining chemical shifts and relaxation rates, we can show an intramolecular complex of C1-RA. GTP-Ras titration and binding to RA disrupts this complex and displaces the C1 domain. Once the C1 domain tumbles freely in solution, a lipid binding interface becomes accessible. Furthermore, we provide evidence of phosphatidylinositol 3-phosphate binding of the free C1 domain.

PubMed: 16698549
DOI: 10.1016/j.str.2006.03.008

実験手法

SOLUTION NMR