2FN9

Thermotoga maritima Ribose Binding Protein Unliganded Form

2FN9 の概要

• エントリーDOI: 10.2210/pdb2fn9/pdb
• 関連するPDBエントリー: 2FN8 2FNC
• 分子名称: ribose ABC transporter, periplasmic ribose-binding protein (2 entities in total)
• 機能のキーワード: rbp, ribose binding protein, periplasmic binding protein, thermophilic proteins, sugar binding protein
• 由来する生物種: Thermotoga maritima
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 64404.86

構造登録者

Cuneo, M.J.,Changela, A.,Tian, Y.,Beese, L.S.,Hellinga, H.W. (登録日: 2006-01-10, 公開日: 2007-01-16, 最終更新日: 2023-08-30)

主引用文献

Cuneo, M.J.,Beese, L.S.,Hellinga, H.W.
Ligand-induced conformational changes in a thermophilic ribose-binding protein.
Bmc Struct.Biol., 8:50-50, 2008

PubMed Abstract: Members of the periplasmic binding protein (PBP) superfamily are involved in transport and signaling processes in both prokaryotes and eukaryotes. Biological responses are typically mediated by ligand-induced conformational changes in which the binding event is coupled to a hinge-bending motion that brings together two domains in a closed form. In all PBP-mediated biological processes, downstream partners recognize the closed form of the protein. This motion has also been exploited in protein engineering experiments to construct biosensors that transduce ligand binding to a variety of physical signals. Understanding the mechanistic details of PBP conformational changes, both global (hinge bending, twisting, shear movements) and local (rotamer changes, backbone motion), therefore is not only important for understanding their biological function but also for protein engineering experiments.

PubMed: 19019243
DOI: 10.1186/1472-6807-8-50

実験手法

X-RAY DIFFRACTION (1.4 Å)