2FI7

Crystal Structure of PilF : Functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa

2FI7 の概要

• エントリーDOI: 10.2210/pdb2fi7/pdb
• 分子名称: type 4 fimbrial biogenesis protein PilF (2 entities in total)
• 機能のキーワード: pilf, tpr, pseudomonas aeruginosa, type 4 pilus, protein binding
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 60129.39

構造登録者

Kim, K. (登録日: 2005-12-28, 公開日: 2006-06-28, 最終更新日: 2024-03-13)

主引用文献

Kim, K.,Oh, J.,Han, D.,Kim, E.E.,Lee, B.,Kim, Y.
Crystal structure of PilF: functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa
Biochem.Biophys.Res.Commun., 340:1028-1038, 2006

PubMed Abstract: PilF is a requisite protein involved in the type 4 pilus biogenesis system from the Gram-negative human pathogenic bacteria, Pseudomonas aeruginosa. We determined the PilF structure at a 2.2A resolution; this includes six tandem tetratrico peptide repeat (TPR) units forming right-handed superhelix. PilF structure was similar to the heat shock protein organizing protein, which interacts with the C-terminal peptide of Hsp90 and Hsp70 via a concave Asn ladder in the inner groove of TPR superhelix. After simulated screening, the C-terminal pentapeptides of PilG, PilU, PilY, and PilZ proved to be a likely candidate binding to PilF, which are ones of 25 necessary components involved in the type 4 pilus biogenesis system. We proposed that PilF would be critical as a bridgehead in protein-protein interaction and thereby, PilF may bind a necessary molecule in type 4 pilus biogenesis system such as PilG, PilU, PilY, and PilZ.

PubMed: 16403447
DOI: 10.1016/j.bbrc.2005.12.108

実験手法

X-RAY DIFFRACTION (2.2 Å)