2FG0

Crystal structure of a putative gamma-d-glutamyl-l-diamino acid endopeptidase (npun_r0659) from nostoc punctiforme pcc 73102 at 1.79 A resolution

2FG0 の概要

• エントリーDOI: 10.2210/pdb2fg0/pdb
• 分子名称: COG0791: Cell wall-associated hydrolases (invasion-associated proteins), GLYCEROL (3 entities in total)
• 機能のキーワード: nlpc/p60 family, structural genomics, joint center for structural genomics, jcsg, protein structure initiative, psi-2, hydrolase
• 由来する生物種: Nostoc punctiforme
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 55458.40

構造登録者

Joint Center for Structural Genomics (JCSG) (登録日: 2005-12-20, 公開日: 2006-01-10, 最終更新日: 2024-11-13)

主引用文献

Xu, Q.,Sudek, S.,McMullan, D.,Miller, M.D.,Geierstanger, B.,Jones, D.H.,Krishna, S.S.,Spraggon, G.,Bursalay, B.,Abdubek, P.,Acosta, C.,Ambing, E.,Astakhova, T.,Axelrod, H.L.,Carlton, D.,Caruthers, J.,Chiu, H.J.,Clayton, T.,Deller, M.C.,Duan, L.,Elias, Y.,Elsliger, M.A.,Feuerhelm, J.,Grzechnik, S.K.,Hale, J.,Won Han, G.,Haugen, J.,Jaroszewski, L.,Jin, K.K.,Klock, H.E.,Knuth, M.W.,Kozbial, P.,Kumar, A.,Marciano, D.,Morse, A.T.,Nigoghossian, E.,Okach, L.,Oommachen, S.,Paulsen, J.,Reyes, R.,Rife, C.L.,Trout, C.V.,van den Bedem, H.,Weekes, D.,White, A.,Wolf, G.,Zubieta, C.,Hodgson, K.O.,Wooley, J.,Deacon, A.M.,Godzik, A.,Lesley, S.A.,Wilson, I.A.
Structural Basis of Murein Peptide Specificity of a gamma-D-Glutamyl-L-Diamino Acid Endopeptidase.
Structure, 17:303-313, 2009

PubMed Abstract: The crystal structures of two homologous endopeptidases from cyanobacteria Anabaena variabilis and Nostoc punctiforme were determined at 1.05 and 1.60 A resolution, respectively, and contain a bacterial SH3-like domain (SH3b) and a ubiquitous cell-wall-associated NlpC/P60 (or CHAP) cysteine peptidase domain. The NlpC/P60 domain is a primitive, papain-like peptidase in the CA clan of cysteine peptidases with a Cys126/His176/His188 catalytic triad and a conserved catalytic core. We deduced from structure and sequence analysis, and then experimentally, that these two proteins act as gamma-D-glutamyl-L-diamino acid endopeptidases (EC 3.4.22.-). The active site is located near the interface between the SH3b and NlpC/P60 domains, where the SH3b domain may help define substrate specificity, instead of functioning as a targeting domain, so that only muropeptides with an N-terminal L-alanine can bind to the active site.

PubMed: 19217401
DOI: 10.1016/j.str.2008.12.008

実験手法

X-RAY DIFFRACTION (1.79 Å)