2FFA

Crystal structure of ABC-ATPase H662A of the ABC-transporter HlyB in complex with ADP

2FFA の概要

• エントリーDOI: 10.2210/pdb2ffa/pdb
• 関連するPDBエントリー: 1MT0 1XEF 2FF7 2FFB
• 分子名称: Alpha-hemolysin translocation ATP-binding protein hlyB, ADENOSINE-5'-DIPHOSPHATE, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (4 entities in total)
• 機能のキーワード: abc-transporter, atpase, transport protein
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cell inner membrane; Multi-pass membrane protein (Probable): P08716
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28231.16

構造登録者

Zaitseva, J.,Oswald, C.,Jumpertz, T.,Jenewein, S.,Holland, I.B.,Schmitt, L. (登録日: 2005-12-19, 公開日: 2006-08-08, 最終更新日: 2023-08-30)

主引用文献

Zaitseva, J.,Oswald, C.,Jumpertz, T.,Jenewein, S.,Wiedenmann, A.,Holland, I.B.,Schmitt, L.
A structural analysis of asymmetry required for catalytic activity of an ABC-ATPase domain dimer.
Embo J., 25:3432-3443, 2006

PubMed Abstract: The ATP-binding cassette (ABC)-transporter haemolysin (Hly)B, a central element of a Type I secretion machinery, acts in concert with two additional proteins in Escherichia coli to translocate the toxin HlyA directly from the cytoplasm to the exterior. The basic set of crystal structures necessary to describe the catalytic cycle of the isolated HlyB-NBD (nucleotide-binding domain) has now been completed. This allowed a detailed analysis with respect to hinge regions, functionally important key residues and potential energy storage devices that revealed many novel features. These include a structural asymmetry within the ATP dimer that was significantly enhanced in the presence of Mg2+, indicating a possible functional asymmetry in the form of one open and one closed phosphate exit tunnel. Guided by the structural analysis, we identified two amino acids, closing one tunnel by an apparent salt bridge. Mutation of these residues abolished ATP-dependent cooperativity of the NBDs. The implications of these new findings for the coupling of ATP binding and hydrolysis to functional activity are discussed.

PubMed: 16858415
DOI: 10.1038/sj.emboj.7601208

実験手法

X-RAY DIFFRACTION (1.7 Å)