2FEI

Solution structure of the second SH3 domain of Human CMS protein

2FEI の概要

• エントリーDOI: 10.2210/pdb2fei/pdb
• 分子名称: CD2-associated protein (1 entity in total)
• 機能のキーワード: cms sh3 domain, structural protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm, cytoskeleton (By similarity): Q9Y5K6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7776.66

構造登録者

Yao, B.,Dai, H.,Jiao, Y.,Wu, J.,Shi, Y. (登録日: 2005-12-15, 公開日: 2006-12-05, 最終更新日: 2024-05-29)

主引用文献

Yao, B.,Zhang, J.,Dai, H.,Sun, J.,Jiao, Y.,Tang, Y.,Wu, J.,Shi, Y.
Solution structure of the second SH3 domain of human CMS and a newly identified binding site at the C-terminus of c-Cbl
Biochim.Biophys.Acta, 1774:35-43, 2007

PubMed Abstract: CMS, cas ligand with multiple Src homology 3 (SH3) domains, belongs to a family of ubiquitously expressed adaptor proteins. Among the CMS binding proteins, c-Cbl has been mostly extensively studied. It was reported that the motif PKPFPR (residues 824-829) of c-Cbl can bind to the N-terminus SH3 domains of CMS. Here we report the solution structure of the second SH3 domain of CMS (CMS_SH3_B), furthermore, we have identified that a peptide from residues 701 to 714 of c-Cbl (Cbl-p), i.e. MTPSSRPLRPLDTS, can specially bind to CMS_SH3_B using NMR chemical shift perturbation, suggesting that the peptide is a new potential CMS binding site. Among the peptide, TPSSRPLR is the core binding motif and Arg709 plays a key role in the interaction. Cbl-p binding interface on CMS_SH3_B along a hydrophobic channel is composed of RT loop, n-Src loop and beta4 strand and divided into three pockets. This work indicates the solution structure of CMS_SH3_B bears the canonical beta-beta-beta-beta-alpha-beta fold and a new binding site in c-Cbl involved in its interaction with CMS, which probably contributes to the clustering of CMS. All the information provided here should be beneficial for the future functional study of CMS.

PubMed: 17188587
DOI: 10.1016/j.bbapap.2006.09.018

実験手法

SOLUTION NMR