2FD4
Crystal Structure of AvrPtoB (436-553)
Summary for 2FD4
| Entry DOI | 10.2210/pdb2fd4/pdb |
| Descriptor | avirulence protein AvrptoB (2 entities in total) |
| Functional Keywords | pseudomonas, avrptob, ring finger, u-box, ubiquitin ligase, ligase |
| Biological source | Pseudomonas syringae |
| Cellular location | Secreted : Q8RSY1 |
| Total number of polymer chains | 1 |
| Total formula weight | 13286.79 |
| Authors | Janjusevic, R.,Stebbins, C.E. (deposition date: 2005-12-13, release date: 2005-12-27, Last modification date: 2024-02-14) |
| Primary citation | Janjusevic, R.,Abramovitch, R.B.,Martin, G.B.,Stebbins, C.E. A bacterial inhibitor of host programmed cell death defenses is an E3 ubiquitin ligase. Science, 311:222-226, 2006 Cited by PubMed Abstract: The Pseudomonas syringae protein AvrPtoB is translocated into plant cells, where it inhibits immunity-associated programmed cell death (PCD). The structure of a C-terminal domain of AvrPtoB that is essential for anti-PCD activity reveals an unexpected homology to the U-box and RING-finger components of eukaryotic E3 ubiquitin ligases, and we show that AvrPtoB has ubiquitin ligase activity. Mutation of conserved residues involved in the binding of E2 ubiquitin-conjugating enzymes abolishes this activity in vitro, as well as anti-PCD activity in tomato leaves, which dramatically decreases virulence. These results show that Pseudomonas syringae uses a mimic of host E3 ubiquitin ligases to inactivate plant defenses. PubMed: 16373536DOI: 10.1126/science.1120131 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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