2FD4

Crystal Structure of AvrPtoB (436-553)

2FD4 の概要

• エントリーDOI: 10.2210/pdb2fd4/pdb
• 分子名称: avirulence protein AvrptoB (2 entities in total)
• 機能のキーワード: pseudomonas, avrptob, ring finger, u-box, ubiquitin ligase, ligase
• 由来する生物種: Pseudomonas syringae
• 細胞内の位置: Secreted : Q8RSY1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13286.79

構造登録者

Janjusevic, R.,Stebbins, C.E. (登録日: 2005-12-13, 公開日: 2005-12-27, 最終更新日: 2024-02-14)

主引用文献

Janjusevic, R.,Abramovitch, R.B.,Martin, G.B.,Stebbins, C.E.
A bacterial inhibitor of host programmed cell death defenses is an E3 ubiquitin ligase.
Science, 311:222-226, 2006

PubMed Abstract: The Pseudomonas syringae protein AvrPtoB is translocated into plant cells, where it inhibits immunity-associated programmed cell death (PCD). The structure of a C-terminal domain of AvrPtoB that is essential for anti-PCD activity reveals an unexpected homology to the U-box and RING-finger components of eukaryotic E3 ubiquitin ligases, and we show that AvrPtoB has ubiquitin ligase activity. Mutation of conserved residues involved in the binding of E2 ubiquitin-conjugating enzymes abolishes this activity in vitro, as well as anti-PCD activity in tomato leaves, which dramatically decreases virulence. These results show that Pseudomonas syringae uses a mimic of host E3 ubiquitin ligases to inactivate plant defenses.

PubMed: 16373536
DOI: 10.1126/science.1120131

実験手法

X-RAY DIFFRACTION (1.8 Å)