2FB4

DIR PRIMAERSTRUKTUR DES KRISTALLISIERBAREN MONOKLONALEN IMMUNOGLOBULINS IGG1 KOL. II. AMINOSAEURESEQUENZ DER L-KETTE, LAMBDA-TYP, SUBGRUPPE I (GERMAN)

Replaces:  1FB4

Summary for 2FB4

• Entry DOI: 10.2210/pdb2fb4/pdb
• Descriptor: IGG1-LAMBDA KOL FAB (LIGHT CHAIN), IGG1-LAMBDA KOL FAB (HEAVY CHAIN) (3 entities in total)
• Functional Keywords: immunoglobulin
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 47142.49

Authors

Marquart, M.,Huber, R. (deposition date: 1989-04-18, release date: 1989-07-12, Last modification date: 2024-11-20)

Primary citation

Kratzin, H.D.,Palm, W.,Stangel, M.,Schmidt, W.E.,Friedrich, J.,Hilschmann, N.
The primary structure of crystallizable monoclonal immunoglobulin IgG1 Kol. II. Amino acid sequence of the L-chain, gamma-type, subgroup I
Biol.Chem.Hoppe-Seyler, 370:263-272, 1989

PubMed Abstract: The immunoglobulin Kol was the first intact antibody molecule which was characterized by high-resolution X-ray crystallography. Furthermore the complete amino-acid sequence of the heavy (H)-chain is known. Here we report the complete amino-acid sequence of the light (L)-chain of the monoclonal immunoglobulin Kol (IgG1). The polypeptide has an Mr of 22,781, consists of 216 amino acids and due to its structure is of the lambda-type. With the characteristic amino acids threonine, asparagine, threonine, glycine and lysine in positions 101, 114, 116, 154, and 165, respectively the Kol L-chain is of the Mcg isotype. With the proteins Mcg, Mot, Bur, Loc and Mem six myeloma-derived amino-acid sequences of the same isotype are known. The amino-acid sequence of the N-terminal variable part is characteristic of subgroup 1. This contribution completes the primary structure of IgG1 Kol.

PubMed: 2713105

Experimental method

X-RAY DIFFRACTION (1.9 Å)