2F87

Solution structure of a GAAG tetraloop in SRP RNA from Pyrococcus furiosus

Summary for 2F87

• Entry DOI: 10.2210/pdb2f87/pdb
• NMR Information: BMRB: 10014
• Descriptor: SRP RNA (1 entity in total)
• Functional Keywords: srp, gnrr, rna
• Total number of polymer chains: 1
• Total formula weight: 3906.40

Authors

Okada, K.,Takahashi, M.,Sakamoto, T.,Nakamura, K.,Kanai, A.,Kawai, G (deposition date: 2005-12-02, release date: 2006-08-08, Last modification date: 2024-05-29)

Primary citation

Okada, K.,Takahashi, M.,Sakamoto, T.,Kawai, G.,Nakamura, K.,Kanai, A.
Solution structure of a GAAG tetraloop in helix 6 of SRP RNA from Pyrococcus furiosus
Nucleosides Nucleotides Nucleic Acids, 25:383-395, 2006

PubMed Abstract: The NMR structure of a 12-mer RNA derived from the helix 6 of SRP RNA from Pyrococcus furiosus, whose loop-closing base pair is U.G, was determined, and the structural and thermodynamic properties of the RNA were compared with those of a mutant RNA with the C:G closing base pair. Although the structures of the two RNAs are similar to each other and adopt the GNRR motif the conformational stabilities are significantly different to each other It was suggested that weaker stacking interaction of the GAAG loop with the U:G closing base pair in 12-mer RNA causes the lower conformational stability.

PubMed: 16838833
DOI: 10.1080/15257770600683979

Experimental method

SOLUTION NMR