2F87

Solution structure of a GAAG tetraloop in SRP RNA from Pyrococcus furiosus

2F87 の概要

• エントリーDOI: 10.2210/pdb2f87/pdb
• NMR情報: BMRB: 10014
• 分子名称: SRP RNA (1 entity in total)
• 機能のキーワード: srp, gnrr, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3906.40

構造登録者

Okada, K.,Takahashi, M.,Sakamoto, T.,Nakamura, K.,Kanai, A.,Kawai, G (登録日: 2005-12-02, 公開日: 2006-08-08, 最終更新日: 2024-05-29)

主引用文献

Okada, K.,Takahashi, M.,Sakamoto, T.,Kawai, G.,Nakamura, K.,Kanai, A.
Solution structure of a GAAG tetraloop in helix 6 of SRP RNA from Pyrococcus furiosus
Nucleosides Nucleotides Nucleic Acids, 25:383-395, 2006

PubMed Abstract: The NMR structure of a 12-mer RNA derived from the helix 6 of SRP RNA from Pyrococcus furiosus, whose loop-closing base pair is U.G, was determined, and the structural and thermodynamic properties of the RNA were compared with those of a mutant RNA with the C:G closing base pair. Although the structures of the two RNAs are similar to each other and adopt the GNRR motif the conformational stabilities are significantly different to each other It was suggested that weaker stacking interaction of the GAAG loop with the U:G closing base pair in 12-mer RNA causes the lower conformational stability.

PubMed: 16838833
DOI: 10.1080/15257770600683979

実験手法

SOLUTION NMR