2F5U

Structural Characterization of the UL25 DNA Packaging Protein from Herpes Simplex Virus Type 1

2F5U の概要

• エントリーDOI: 10.2210/pdb2f5u/pdb
• 分子名称: Virion protein UL25 (2 entities in total)
• 機能のキーワード: hsv-1, capsid protein, dna packaging, ul25, head completion, viral protein
• 由来する生物種: Human herpesvirus 1 (Herpes simplex virus type 1)
• 細胞内の位置: Virion: P10209
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 48433.92

構造登録者

Bowman, B.R. (登録日: 2005-11-27, 公開日: 2006-02-28, 最終更新日: 2024-02-14)

主引用文献

Bowman, B.R.,Welschhans, R.L.,Jayaram, H.,Stow, N.D.,Preston, V.G.,Quiocho, F.A.
Structural characterization of the UL25 DNA-packaging protein from herpes simplex virus type 1
J.Virol., 80:2309-2317, 2006

PubMed Abstract: Herpesviruses replicate their double stranded DNA genomes as high-molecular-weight concatemers which are subsequently cleaved into unit-length genomes by a complex mechanism that is tightly coupled to DNA insertion into a preformed capsid structure, the procapsid. The herpes simplex virus type 1 UL25 protein is incorporated into the capsid during DNA packaging, and previous studies of a null mutant have demonstrated that its function is essential at the late stages of the head-filling process, either to allow packaging to proceed to completion or for retention of the viral genome within the capsid. We have expressed and purified an N-terminally truncated form of the 580-residue UL25 protein and have determined the crystallographic structure of the region corresponding to amino acids 134 to 580 at 2.1-Angstroms resolution. This structure, the first for any herpesvirus protein involved in processing and packaging of viral DNA, reveals a novel fold, a distinctive electrostatic distribution, and a unique "flexible" architecture in which numerous flexible loops emanate from a stable core. Evolutionary trace analysis of UL25 and its homologues in other herpesviruses was used to locate potentially important amino acids on the surface of the protein, leading to the identification of four putative docking regions for protein partners.

PubMed: 16474137
DOI: 10.1128/JVI.80.5.2309-2317.2006

実験手法

X-RAY DIFFRACTION (2.1 Å)